GC-MS analysis of propolis of Indian origin

作者：N Kumar, KK Mueen Ahmed, R Dang, T. N. Shivananda, Kusal K. Das · 发表于：Journal of Young Pharmacists · 年份：2009 · DOI：10.4103/0975-1483.51876 · 被引用次数：16 · 研究领域：Bee Products Chemical Analysis、Essential Oils and Antimicrobial Activity、Phytochemistry Medicinal Plant Applications

More than 300 constituents have been identiÞ ed in different propolis samples. This study investigated the composition of ethanolic extracts of propolis samples collected from the Gujarat zone. A gas chromatographymass spectrometry was carried out on a Agilent GC-MS 5975 under electron impact ionization (70 eV). The chromatographic column for the analysis was done by the HP5MS capillary column (30 m x 0.25 mm internal diameter). The carrier gas used was helium at a s ow rate of 1 ml/min. The oven temperature was 100°C to 280°C with a constant increase of 10°C. Propolis samples were analyzed with the column held initially at 60°C for 2 min and then increased to 230°C with a 2°C/ min heating ramp and then kept at 230°C for 3 min. Finally, the temperature was increased to 280°C with a 3°C/min heating ramp. The injection was performed in split mode at 220°C. Sample volumes of 1 μl were injected and analyzed by GC-MS. The following compounds were identiÞ ed for the Þ rst time in the propolis sample: p-coumeric acid, Benzyl cinnamate, 4-pentanoic acid, and Ferulic acid. The main type of the compound identiÞ ed was fatty acids derivatives. Similar results were found by Silici, et al. [5] for the turkey propolis. Key words: GC-MS analysis, propolis, Honeycomb .