Induction of fibroblast proliferation by interleukin‐1 derived from human monocytic leukemia cells

作者：Arnold E. Postlethwaite, Lawrence B. Lachman, Andrew H. Kang · 发表于：Arthritis & Rheumatism · 年份：1984 · DOI：10.1002/art.1780270905 · 被引用次数：124 · 研究领域：Immune cells in cancer、Immune Response and Inflammation、Hematopoietic Stem Cell Transplantation

Human interleukin-1 (IL-1), free of contaminating lymphokines, was isolated from cultures of purified monoblasts from a patient with acute monocytic leukemia. Partially purified IL-1 (diafiltration, ultrafiltration, and isoelectric focusing) stimulated proliferation of subconfluent human fibroblasts in vitro. Further purification of IL-1 by high-resolution gel filtration- and anion exchange-high performance liquid chromatography revealed that fibroblast proliferation activity could not be separated from IL-1 activity (thymocyte proliferation), suggesting that both activities are the properties of a single molecule. Fibroblasts and thymocytes exhibited a similar sensitivity to the proliferative effects of IL-1. These findings suggest that macrophages participating in inflammatory reactions in vivo might release IL-1, which could function to expand fibroblast populations at sites of inflammatory reactions, by acting as a fibroblast growth factor.