Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: Acceleration of the renaturation rate

作者：June Faith R. Escara, James R. Hutton · 发表于：Biopolymers · 年份：1980 · DOI：10.1002/bip.1980.360190708 · 被引用次数：282 · 研究领域：DNA and Nucleic Acid Chemistry、Electrostatics and Colloid Interactions、RNA Interference and Gene Delivery

Abstract The thermal stability and renaturation kinetics of DNA have been studied as a function of dimethyl sulfoxide (DMSO) concentration. Increasing the concentration of DMSO lowers the melting temperature of DNA but results in an increased second‐order renaturation rate. For example, in a DNA solution containing 0.20M NaCl, 0.01M Tris (pH 8.0), and 0.001M EDTA, the addition of 40% DMSO lowers the melting temperature of the DNA by 27°C and approximately doubles the optimal renaturation rate. The effect of DMSO on the renaturation rate is shown to be at least partially due to its effect on the solution dielectric constant and to be consistent with the polyelectrolyte counterion condensation theory of Manning [(1976) Biopolymers 15, 1333–1343].