Comparison of the Effects of Viscum album Lectin ML-1 and Fresh Plant Extract (Isorel) on the Cell Growth in vitro and Tumorigenicity of Melanoma B16F10

作者：Neven Žarković, Tea Kališnik, Iva Lončarić, Suzana Borović, Susanne Mang, Dieter Kissel, M. Konitzer, Mislav Jurin, Serife Grainza · 发表于：Cancer Biotherapy and Radiopharmaceuticals · 年份：1998 · DOI：10.1089/cbr.1998.13.121 · 被引用次数：25 · 研究领域：Toxin Mechanisms and Immunotoxins、Transgenic Plants and Applications、Plant Parasitism and Resistance

Numerous findings indicate that specific plant lectins acting against cancer could be major active components of Viscum album extracts, although activity of low molecular weight components (peptides, carbohydrates and alkaloids) might be as essential for the beneficial activity of the plain plant extracts, too. Thus, active principle of Viscum album extracts is still not understood, and is difficult to be analysed because of the complex composition of the extracts and uncertainty of the standardised effectiveness (batch consistency) of the extracts. The aims of this study were to compare the concentration dependent effects of the pure mistletoe lectin (ML-1) with the fresh plant Viscum album extract (Isorel) and its different MW components on the in vitro growth of ConA stimulated lymphocytes, on the growth and tumorigenicity (artificial lung metastases development) of murine melanoma B16F10 cells, and to compare concentration dependent effects of the different types of the Viscum album extracts in vitro (applying novel type of MTT assay). The results obtained indicate that the effects of Isorel used at high dose could be result of toxic activity of the mistletoe lectins ("ML-1 like" activity). Unlike ML-1, if used at low concentrations, Isorel selectively inhibited tumor cells, due the activity of the low MW components. On the other hand, the number of tumor nodules was reduced (in comparison to the control) equally in the lungs of mice injected with B16F10 cells pre-treated...