Inhibition of Cellular Proliferation by Antisense Oligodeoxynucleotides to PCNA Cyclin

作者：Dariusz Jaskulski, J. Kim deRiel, W. Edward Mercer, Bruno Calabretta, Renato Baserga · 发表于：Science · 年份：1988 · DOI：10.1126/science.2897717 · 被引用次数：427 · 研究领域：DNA and Nucleic Acid Chemistry、Molecular Biology Techniques and Applications、Biopolymer Synthesis and Applications

The proliferating cell nuclear antigen (PCNA or cyclin) is a nuclear protein recently identified as a cofactor of DNA polymerase delta. When exponentially growing Balb/c3T3 cells are exposed to antisense oligodeoxynucleotides to PCNA, both DNA synthesis and mitosis are completely suppressed. A corresponding sense oligodeoxynucleotide has no inhibitory effects. These experiments indicate that PCNA (cyclin) is important in cellular DNA synthesis and in cell cycle progression.