Improvement of the Enzyme Performance of Trypsin via Adsorption in Mesoporous Silica SBA-15: Hydrolysis of BAPNA

作者：Shanshan Li, Zhuofu Wu, Ming Lu, Zhi Wang, Zhengqiang Li · 发表于：Molecules · 年份：2013 · DOI：10.3390/molecules18011138 · 被引用次数：23 · 研究领域：Mesoporous Materials and Catalysis、Electrochemical sensors and biosensors、Enzyme Catalysis and Immobilization

The enzymatic performance of trypsin in hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) was improved by adsorption on Santa Barbara Amorphous (SBA)-15 mesoporous silica. The optimal immobilization conditions were screened and the properties of immobilized enzyme have also been studied. Under the optimal conditions, the immobilized trypsin displays maximum specific activity (49.8 μmol/min/g). The results also indicate that the immobilized trypsin exhibits better storage stability.