A consensus protocol for the standardization of cerebrospinal fluid collection and biobanking

作者：Charlotte E. Teunissen, Axel Petzold, Jeffrey L. Bennett, Frode S. Berven, Lou Brundin, Manuel Comabella, D. Franciotta, Jette Lautrup Frederiksen, J. O. Fleming, Roberto Furlan, Rogier Q. Hintzen, Steven G. Hughes, M. H. Johnson, Eva Krasulová, Jens Kühle, Maria Chiara Magnone, Cecília Rajda, Konrad Rejdak, Hollie Schmidt, Vincent Van Pesch, Emmanuelle Waubant, Christian Wolf, Gavin Giovannoni, Bernhard Hemmer, Hayrettin Tumani, Florian Deisenhammer · 发表于：Neurology · 年份：2009 · DOI：10.1212/wnl.0b013e3181c47cc2 · 被引用次数：872 · 研究领域：Multiple Sclerosis Research Studies、Neuroinflammation and Neurodegeneration Mechanisms、S100 Proteins and Annexins

There is a long history of research into body fluid biomarkers in neurodegenerative and neuroinflammatory diseases. However, only a few biomarkers in CSF are being used in clinical practice. One of the most critical factors in CSF biomarker research is the inadequate powering of studies because of the lack of sufficient samples that can be obtained in single-center studies. Therefore, collaboration between investigators is needed to establish large biobanks of well-defined samples. Standardized protocols for biobanking are a prerequisite to ensure that the statistical power gained by increasing the numbers of CSF samples is not compromised by preanalytical factors. Here, a consensus report on recommendations for CSF collection and biobanking is presented, formed by the BioMS-eu network for CSF biomarker research in multiple sclerosis. We focus on CSF collection procedures, preanalytical factors, and high-quality clinical and paraclinical information. The biobanking protocols are applicable for CSF biobanks for research targeting any neurologic disease.