Transient Expression of Chimeric Genes Delivered into Pollen by Microprojectile Bombardment

作者：David Twell, Theodore M. Klein, Michael Fromm, Sheila McCormick · 发表于：PLANT PHYSIOLOGY · 年份：1989 · DOI：10.1104/pp.91.4.1270 · 被引用次数：201 · 研究领域：Transgenic Plants and Applications、Plant tissue culture and regeneration、Plant Reproductive Biology

Chimeric genes containing a pollen-specific promoter from tomato (Lycopersicon esculentum) or the CaMV35S promoter were transiently expressed following their introduction into tobacco (Nicotiana tabacum) pollen using high velocity microprojectiles. Transient expression of the microprojectile-introduced genes in leaves and pollen was similar to that observed for these genes in stably transformed tobacco plants.