Molecular cloning and analysis of cDNA sequences derived from polyA+RNA from barley endosperm: identification of B hordein related clones

作者：Brian Forde, Martin Ernst Kreis, M. Bahman Bahramian, Jayne A. Matthews, Ben J. Miflin, Richard D. Thompson, Dorothea Bartels, Richard Bailey Flavell · 发表于：Nucleic Acids Research · 年份：1981 · DOI：10.1093/nar/9.24.6689 · 被引用次数：97 · 研究领域：Wheat and Barley Genetics and Pathology、Phytase and its Applications、Plant-Microbe Interactions and Immunity

A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.