Clustering of RNA polymerase B molecules in the 5′ moiety of the adult β-globin gene of hen erythrocytes

作者：Patricio Gariglio, María Elena de Bellard, Pierre Chambon · 发表于：Nucleic Acids Research · 年份：1981 · DOI：10.1093/nar/9.11.2589 · 被引用次数：174 · 研究领域：Genomics and Chromatin Dynamics、Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities、RNA modifications and cancer

Nuclei were prepared from mature and immature hen erythrocytes and incubated for RNA synthesis in the absence or in the presence of Sarkosyl. The in vitro labelled synthesized RNA was hybridized to specific 5' and 3' fragments of the chicken adult beta-globin gene to investigate the possible presence of RNA polymerase molecules bound to this gene in the form of transcriptional complexes. Surprisingly, such RNA polymerase B molecules were found located preferentially in the 5' end moiety of the beta-globin genes of mature erythrocytes, although they are apparently evenly distributed along the beta-globin genes of immature polychromatic erythrocytes. The significance of these observations with respect to (1) preferential DNaseI sensitivity of "genes which have been transcribed" and (2) control of transcription in eukaryotic cells is discussed.