Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.

作者：R. A. Hoffman, Patrick C. Kung, W. Hansen, Gideon Goldstein · 发表于：Proceedings of the National Academy of Sciences · 年份：1980 · DOI：10.1073/pnas.77.8.4914 · 被引用次数：450 · 研究领域：Monoclonal and Polyclonal Antibodies Research、T-cell and B-cell Immunology、Glycosylation and Glycoproteins Research

A simple and rapid method for the determination of human T lymphocyte subclasses in buffy coat preparations or whole blood is described. This technique uses flow cytometry to distinguish lymphocytes from other leukocytes on the basis of their light-scattering properties. Lymnphocyte subclasses were enumerated by cellular immunofluorescence; the immunofluorescent signals were produced by monoclonal antibodies to surface differentiation antigens on T cells. Conventional techniques of enumerating T lymphocyte subclasses entail time-consuming (up to 2 days) density gradient and E rosette enrichement, and require at least 20 ml of blood. The method described here uses as little as 50 microliters of whole blood for each antibody tested and produces results within in 2 hr.