Isolation and Characterization of Novel, Highly Proliferative Human CD34/CD73-Double-Positive Testis-Derived Stem Cells for Cell Therapy

作者：Won Yun Choi, Hwang Gyun Jeon, Young Sun Chung, Jung Jin Lim, Dong Hyuk Shin, Jung Mo Kim, Byeong Seong Ki, Seung‐Hun Song, Seong-Jun Choi, Keun‐Hong Park, Sung Han Shim, Jisook Moon, Sung Jun Jung, Hyun Mi Kang, Seah Park, Hyung‐Min Chung, Jung Jae Ko, Kwang Yul, Tae Ki Yoon, Haekwon Kim, Dong Ryul Lee · 发表于：Stem Cells and Development · 年份：2013 · DOI：10.1089/scd.2012.0385 · 被引用次数：22 · 研究领域：Tissue Engineering and Regenerative Medicine、RNA Interference and Gene Delivery、Pluripotent Stem Cells Research

Human adult stem cells are a readily available multipotent cell source that can be used in regenerative medicine. Despite many advantages, including low tumorigenicity, their rapid senescence and limited plasticity have curtailed their use in cell-based therapies. In this study, we isolated CD34/CD73-double-positive (CD34(+)/CD73(+)) testicular stromal cells (HTSCs) and found that the expression of CD34 was closely related to the cells' stemness and proliferation. The CD34(+)/CD73(+) cells grew in vitro for an extended period of time, yielding a multitude of cells (5.6×10(16) cells) without forming tumors in vivo. They also differentiated into all three germ layer lineages both in vitro and in vivo, produced cartilage more efficiently compared to bone marrow stem cells and, importantly, restored erectile function in a cavernous nerve crush injury rat model. Thus, these HTSCs may represent a promising new autologous cell source for clinical use.