Evaluation of gelatin hydrogel crosslinked with various crosslinking agents as bioadhesives:In vitro study

作者：Hsing‐Wen Sung, Don-Mou Huang, Wen‐Hsiang Chang, Rong Huang, Jer‐Chen Hsu · 发表于：Journal of Biomedical Materials Research · 年份：1999 · DOI：10.1002/(sici)1097-4636(19990915)46:4<520::aid-jbm10>3.0.co;2-9 · 被引用次数：311 · 研究领域：Surgical Sutures and Adhesives、Hemostasis and retained surgical items、Intestinal and Peritoneal Adhesions

Bioadhesives are used for tissue adhesion and hemostasis in surgery. A gelatin-resorcinol mixture crosslinked with formaldehyde (GRF glue) and/or glutaraldehyde (GRG) is used for this purpose. Although the bonding strength of the GRF glue to tissue is satisfactory, concerns about the cytotoxicity of formaldehyde are reported in the literature. It was suggested that the cytotoxicity problem of the GRF glue may be overcome by changing its crosslinking method. The study was therefore undertaken to assess the feasibility of using an epoxy compound (GRE glue), a water-soluble carbodiimide (GAC glue), or genipin (GG glue) to crosslink with a gelatin hydrogel as new bioadhesives. GRF glue and GRG glue were used as controls. The results of our cytotoxicity study suggested that the cellular compatibility of the GAC and GG glues was superior to the GRF, GRG, and GRE glues. The gelation time for the GG glue was relatively longer than the GRF and GRG glues, while no gelation time could be determined for the GAC glue. Additionally, it took approximately 17 h for the GRE glue to become adhesive. The GRF and GRG glues had the greatest bonding strengths to tissue among all test adhesives, while the bonding strengths of the GAC and GG glues were comparable. In contrast, there was almost no bonding strength to tissue for the GRE glue. However, the GRF and GRG glues were less flexible than the GAC and GG glues. Subsequent to the bonding strength measurement, each test adhesive was found to adhe...