Nuclease Activities in a Complex of Human Recombination and DNA Repair Factors Rad50, Mre11, and p95

作者：Kelly M. Trujillo, Shyng‐Shiou F. Yuan, Eva Y.-H.P. Lee, Patrick Sung · 发表于：Journal of Biological Chemistry · 年份：1998 · DOI：10.1074/jbc.273.34.21447 · 被引用次数：417 · 研究领域：DNA Repair Mechanisms、Plant Genetic and Mutation Studies、Carcinogens and Genotoxicity Assessment

Genetic studies in yeast have indicated a role of the RAD50 and MRE11 genes in homologous recombination, telomere length maintenance, and DNA repair processes. Here, we purify from nuclear extract of Raji cells a complex consisting of human Rad50, Mre11, and another protein factor with a size of about 95 kDa (p95), which is likely to be Nibrin, the protein encoded by the gene mutated in Nijmegen breakage syndrome. We show that the Rad50-Mre11-p95 complex possesses manganese-dependent single-stranded DNA endonuclease and 3' to 5' exonuclease activities. These nuclease activities are likely to be important for recombination, repair, and genomic stability.