Phenotypic changes of human neuroblastoma cells in culture induced by 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate

作者：Sven Påhlman, Lena Odelstad, Erik Larsson, Gunnar Grotte, Kenneth Nilsson · 发表于：International Journal of Cancer · 年份：1981 · DOI：10.1002/ijc.2910280509 · 被引用次数：261 · 研究领域：Ubiquitin and proteasome pathways、Cancer, Hypoxia, and Metabolism、Chemical Synthesis and Analysis

SK-N-SH and SH-SY5Y human neuroblastoma cells treated by 12-)-tetradecanoyl-phorbol-13-acetate(TPA) express morphological and biochemical changes, which indicate that differentiation towards more mature cells has occurred. The most prominent morphological changes were the development in 40-60% of the cells of cell-surface projections longer than 50 micrometers and cytoplasmic neurosecretory granules demonstrated by electron microscopy. At the biochemical level, TPA induced a two-fold increase in the relative activity of neuron-specific enolase and 30- to 40-fold increase in noradrenaline and adrenaline concentrations. A decrease in proliferation rate of TPA-treated cells was observed. The biological effects of TPA were slightly potentiated by nerve growth factor.