A new method for sequencing DNA.

作者：Allan M. Maxam, Wendy V. Gilbert · 发表于：Proceedings of the National Academy of Sciences · 年份：1977 · DOI：10.1073/pnas.74.2.560 · 被引用次数：7976 · 研究领域：Chemical Synthesis and Analysis、DNA and Nucleic Acid Chemistry、Advanced biosensing and bioanalysis techniques

DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.