Mutagenesis and Mapping of a Mouse Gene, Clock , Essential for Circadian Behavior

作者：Martha Hotz Vitaterna, David P. King, Anne‐Marie Chang, Jon M. Kornhauser, Phillip L. Lowrey, J. David McDonald, William F. Dove, Lawrence H. Pinto, Fred William Turek, Joseph S. Takahashi · 发表于：Science · 年份：1994 · DOI：10.1126/science.8171325 · 被引用次数：1636 · 研究领域：Circadian rhythm and melatonin、Light effects on plants、Photoreceptor and optogenetics research

In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.