Specific association between the human DNA repair proteins XPA and ERCC1.

作者：Lian Li, Stephen J. Elledge, Carolyn A. Peterson, Elise S. Bales, Randy J. Legerski · 发表于：Proceedings of the National Academy of Sciences · 年份：1994 · DOI：10.1073/pnas.91.11.5012 · 被引用次数：307 · 研究领域：DNA Repair Mechanisms、Genomics and Chromatin Dynamics、Epigenetics and DNA Methylation

Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.