Characterization of Human Platelet Vascular Permeability-Enhancing Activity

作者：Ralph L. Nachman, Babette Barbash Weksler, Barbara Ferris · 发表于：Journal of Clinical Investigation · 年份：1972 · DOI：10.1172/jci106843 · 被引用次数：129 · 研究领域：Platelet Disorders and Treatments、Complement system in diseases、Blood properties and coagulation

A C T Human platelet acid extract obtained from both whole platelets and from isolated subcellular granules was partially purified by DEAE-cellulose chromatography and Sephadex gel filtration.The heat- stable, nondialyzable cationic protein fraction with a mol wt of approximately 30,000 produced a biphasic increase in vascular permeability in rabbit skin and also had antiheparin activity.The acute (15 min) in- crease in vascular permeability was blocked by prior treatment of the animal with antihistamine and was characterized histologically by edema of perivascular tissues and dilation of capillaries and veinules.The de- layed (3 hr) permeability effect was not blocked by antihistamine and was characterized histologically by leukocytic infiltration into the skin.The experiments described suggest that human platelet lysosomal re- lease of cationic proteins may increase vascular per- meability by several mechanisms including endogenous histamine release as well as delayed chemotaxis.not contain histamine, serotonin, kallikrein, plasmin, C! or PF/dil.The present studies were undertaken to analyze in greater detail the cationic permeability pro- tein(s) of human platelets.Human platelet acid extract was partially purified by DEAE-cellulose chromatogra- Dr. Weksler held a U. S. Public Health Service Special Fellowship.