Separation of human neutrophils in self-generated continuous density gradients of percoll.

作者：Nobuhiro Uyesaka, Amy TAJIRI, Koichi Arai · 发表于：The Japanese Journal of Physiology · 年份：1989 · DOI：10.2170/jjphysiol.39.629 · 被引用次数：4 · 研究领域：Neutrophil, Myeloperoxidase and Oxidative Mechanisms、Immune Response and Inflammation、Blood disorders and treatments

Human neutrophils were separated into two fractions using a continuous density gradient of Percoll solution. A marked decrease in O2- production was observed in the low-density neutrophil fraction. There was little erythrocyte contamination in the high-density neutrophil fraction (less than 0.5%), and thus the hypotonic or ammonium chloride lysis of erythrocytes was not necessary.