Different electrophysiological responses of canine endocardium and epicardium to combined hyperkalemia, hypoxia, and acidosis.

作者：Robert F. Gilmour, Douglas Peter Zipes · 发表于：Circulation Research · 年份：1980 · DOI：10.1161/01.res.46.6.814 · 被引用次数：166 · 研究领域：Cardiac Arrest and Resuscitation、Renal function and acid-base balance、Neuroscience of respiration and sleep

To determine whether canine epicardium and endocardium show intrinsically different electrophysiological responses to metabolic alterations that occur during acute myocardial ischemia in vivo, endocardial, epicardial, papillary muscle tip, and Purkinje fibers (PF) were superfused in vitro with Tyrode's solution containing 8.0 DM KC1 at a pH of 6.85 and a Po 2 < 50 mm Hg.During the initial 10 minutes of superfusion with altered Tyrode's solution, reduction of action potential (AP) amplitude and dV/dtmax and prolongation of activation times were greater in epicardium than in endocardium or in PF, despite similar changes in resting membrane potential.After superfusion for 15 minutes, only 3 of 18 epicardial and 5 of 16 papillary muscle cells were excitable, whereas 14 of 16 endocardial muscle cells and 13 of 13 PF still were responsive.Membrane responsiveness at takeoff potentials < -65mV was lower in epicardium and papillary muscle than in endocardium during superfusion with normal or altered Tyrode's solution.The effects produced by the initial 15 minutes of superfusion with altered Tyrode's solution were partially reversed in endocardium and PF but not in epicardium or papillary muscle, during a subsequent 20-to 60-minute period of continued superfusion.Tetrodotoxin (1'1'X, 5 x 10" 6 M) depressed AP amplitude and dV/dtm., more in epicardium than in endocardium.TTX had only a small effect on the upstroke of PF AP, but shortened AP duration by 30-40%.Verapamil (2 x 10~6 M) had ...