O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

作者：J R Benson, P. E. Hare · 发表于：Proceedings of the National Academy of Sciences · 年份：1975 · DOI：10.1073/pnas.72.2.619 · 被引用次数：743 · 研究领域：Chemical Synthesis and Analysis、Click Chemistry and Applications

O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.