Morphological diversity of displaced retinal ganglion cells in the rat: A lucifer yellow study

作者：Edgar Buhl, J.F. Dann · 发表于：The Journal of Comparative Neurology · 年份：1988 · DOI：10.1002/cne.902690206 · 被引用次数：45 · 研究领域：Retinal Development and Disorders、Neuroscience and Neural Engineering、Neuroscience and Neuropharmacology Research

Abstract Displaced retinal ganglion cells (DRGCs) were retrogradely labelled by injections of the fluorescent dye Fast Blue into the superior colliculi of pigmented rats. Following fixation these cells were intracellularly injected with Lucifer Yellow to determine their dendritic morphology and distribution. Graphic reconstruction of Lucifer Yellow‐filled prelabelled neurones revealed a heterogeneous population of DRGCs. Their stratification within the inner plexiform layer was diverse and cells were classified according to their dendritic morphology. The present sample consists largely of unistratifying neurones, the dendrites of which arborized within a narrow sublamina of the inner plexiform layer. They were characterized by a centrally located soma and densely branched dendritic network with little overlap within the branching pattern. In contrast, bistratifying DRGCs possessed a loose and sparsely branched dendritic structure, while diffusely stratifying neurones contained a high degree of dendritic crossing, culminating in a complex network, in which the soma position was biased toward the periphery. One type of DRGC bore a striking resemblance to type 1 neurones (Perry, 1979; Proc. R. Soc. Lond. [Biol.] 204 :363–375) in the ganglion cell layer. They were characterized by a large soma (15.8 μm ± 2.2 μm s.d.) and a dendritic field diameter averaging 288 μm (s.d. ± 62 μm) and were on average larger than the rest of the displaced population but smaller than type 1 cells in...