Mitochondrial respiration in human viable platelets—Methodology and influence of gender, age and storage

作者：Fredrik Sjövall, Johannes K. Ehinger, Sigurður E. Marelsson, Saori Morota, Eleonor Åsander Frostner, Hiroyuki Uchino, Johan Lundgren, Einar Arnbjörnsson, Magnus Joakim Hansson, Vineta Fellman, Eskil Elmér · 发表于：Mitochondrion · 年份：2012 · DOI：10.1016/j.mito.2012.11.001 · 被引用次数：136 · 研究领域：Metabolism and Genetic Disorders、Renal Transplantation Outcomes and Treatments、Neonatal Health and Biochemistry

Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.