Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile

作者：Maja Rupnik, Veit Braun, Florian Soehn, Miha Janc, Monika Hofstetter, Rita Laufenberg–Feldmann, Christoph von Eichel‐Streiber · 发表于：FEMS Microbiology Letters · 年份：2006 · DOI：10.1111/j.1574-6968.1997.tb10288.x · 被引用次数：91 · 研究领域：Clostridium difficile and Clostridium perfringens research、Viral gastroenteritis research and epidemiology、Herbal Medicine Research Studies

We have used six independent polymerase chain reactions (A1-A3 and B1-B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5' third of tcdB (B1 fragment) and/or the 3' third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.