Dichlorophenylurea-insensitive Reduction of Silicomolybdic Acid by Chloroplast Photosystem II

作者：Rita Barr, F. L. Crane, Robert T. Giaquinta · 发表于：PLANT PHYSIOLOGY · 年份：1975 · DOI：10.1104/pp.55.3.460 · 被引用次数：48 · 研究领域：Photosynthetic Processes and Mechanisms、Electrochemical sensors and biosensors、Microbial Fuel Cells and Bioremediation

The photoreduction of silicomolybdate and other heteropoly ions by chloroplasts is insensitive to 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU). Both water and diphenylcarbazide can be used as electron source for the reduction. Three different assays for silicomolybdate reduction are described including oxygen evolution, formation of a reduced heteropoly blue silicomolybdate, or an indirect assay for reduced silicomolybdate by redox indicators, such as ferricyanide or cytochrome c. The effects of detergents and tris washing are consistent with silicomolybdate reduction through photosystem II before the DCMU site. The effects of orthophenanthroline and bathophenanthroline indicate chelator-sensitive sites in photosystem II before the site of DCMU action.