Cloning and sequence of cDNA coding for alpha 1-antitrypsin.

作者：Kotoku Kurachi, T.S. Chandra, S J Degen, Thomas Taylor White, Thomas L. Marchioro, Savio L.C. Woo, Earl W. Davie · 发表于：Proceedings of the National Academy of Sciences · 年份：1981 · DOI：10.1073/pnas.78.11.6826 · 被引用次数：179 · 研究领域：Molecular Biology Techniques and Applications、Machine Learning in Bioinformatics、Antimicrobial Peptides and Activities

Recombinant plasmids containing human and baboon cDNA have been screened for alpha 1-antitrypsin, a major serine protease inhibitor present in blood. One plasmid, designated pBa alpha 1a2, was found to contain a cDNA insert of 1352 base pairs coding for the baboon inhibitor. It included 45 nucleotides that code for 15 amino acids present in the amino-terminal signal sequence of the protein, 1182 nucleotides that code for 394 amino acids in the mature protein, a stop codon, and a noncoding region of 76 nucleotides. Comparison of the amino acid sequences of baboon alpha 1-antitrypsin, human antithrombin III, and chicken ovalbumin indicated that these three proteins are about 230% homologous. A second plasmid, designated pH alpha 1a1, was found to contain a human cDNA insert of 306 base pairs. This plasmid coded for 69 amino acids present in the carboxyl-terminal region of human alpha 1-antitrypsin. The human and baboon cDNAs and their amino acid sequences are greater than 96% homologous.