Enzyme-Immobilized Nanofiltration Membrane To Mitigate Biofouling Based on Quorum Quenching

作者：Jae‐Hyuk Kim, Dong-Chan Choi, Kyung‐Min Yeon, Sang-Ryoung Kim, Chung‐Hak Lee · 发表于：Environmental Science & Technology · 年份：2011 · DOI：10.1021/es103483j · 被引用次数：226 · 研究领域：Membrane Separation Technologies、Graphene and Nanomaterials Applications、Microplastics and Plastic Pollution

Recently, enzymatic quorum quenching (in the form of a free enzyme or an immobilized form on a bead) was successfully applied to a submerged membrane bioreactor with a microfiltration membrane for wastewater treatment as a novel approach to control membrane biofouling. In this study, a quorum quenching enzyme (acylase) was directly immobilized onto a nanofiltration membrane to mitigate biofouling in a nanofiltration process. In a flow cell experiment, the acylase-immobilized membrane with quorum quenching activity prohibited the formation of mushroom-shaped mature biofilm due to the reduced secretion of extracellular polymeric substances (EPS). The acylase-immobilized membrane maintained more than 90% of its initial enzyme activity for more than 20 iterative cycles of reaction and washing procedure. In the lab-scale continuous crossflow nanofiltration system operated at a constant pressure of 2 bar, the flux with the acylase-immobilized nanofiltration (NF) membrane was maintained at more than 90% of its initial flux after a 38-h operation, whereas that with the raw NF membrane decreased to 60% accompanied with severe biofouling. The quorum quenching activity of the acylase-immobilized membrane was also confirmed by visualizing the spatial distribution of cells and polysaccharides on the surface of each membrane using confocal laser scanning microscopy (CLSM) image analysis technique.