Transcriptional profile of Taxus chinensis cells in response to methyl jasmonate

作者：Shutao Li, Peng Zhang, Meng Zhang, Chunhua Fu, Chunfang Zhao, Yanshan Dong, An‐Yuan Guo, Longjiang Yu · 发表于：BMC Genomics · 年份：2012 · DOI：10.1186/1471-2164-13-295 · 被引用次数：109 · 研究领域：Plant Pathogens and Fungal Diseases、Plant biochemistry and biosynthesis、Cancer Treatment and Pharmacology

BACKGROUND: Methyl jasmonate (MeJA) has been successfully used as an effective elicitor to enhance production of taxol and other taxanes in cultured Taxus cells. However the mechanism of MeJA-mediated taxane biosynthesis remains unclear. Genomic information for species in the genus Taxus is currently unavailable. Therefore, information about the transcriptome of Taxus cells and specifically, description of changes in gene expression in response to MeJA, is needed for the better exploration of the biological mechanisms of MeJA-mediated taxane biosynthesis. RESULTS: In this research, the transcriptome profiles of T. chinensis cells at 16 hours (T16) after MeJA treatment and of mock-treated cells (T0) were analyzed by "RNA-seq" to investigate the transcriptional alterations of Taxus cell in response to MeJA elicitation. More than 58 million reads (200 bp in length) of cDNA from both samples were generated, and 46,581 unigenes were found. There were 13,469 genes found to be expressed differentially between the two timepoints, including all of the known jasmonate (JA) biosynthesis/JA signaling pathway genes and taxol-related genes. The qRT-PCR results showed that the expression profiles of 12 randomly selected DEGs and 10 taxol biosynthesis genes were found to be consistent with the RNA-Seq data. MeJA appeared to stimulate a large number of genes involved in several relevant functional categories, such as plant hormone biosynthesis and phenylpropanoid biosynthesis. Additionally, m...