Adhesion Forces Between Individual Ligand-Receptor Pairs

作者：Ernst‐Ludwig Florin, Vincent T. Moy, Hermann E. Gaub · 发表于：Science · 年份：1994 · DOI：10.1126/science.8153628 · 被引用次数：1902 · 研究领域：Force Microscopy Techniques and Applications、Molecular Junctions and Nanostructures、Nanofabrication and Lithography Techniques

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.