Alternative trans -splicing of constant and variable exons of a Drosophila axon guidance gene, lola

作者：Takayuki Horiuchi, Edward Giniger, Toshiro Aigaki · 发表于：Genes & Development · 年份：2003 · DOI：10.1101/gad.1137303 · 被引用次数：88 · 研究领域：RNA Research and Splicing、Developmental Biology and Gene Regulation、Ubiquitin and proteasome pathways

longitudinals lacking (lola) is a complex Drosophila gene encoding at least 20 protein isoforms,each bearing the same N-terminal constant region linked to a different C-terminal variable region. Different isoforms specify different aspects of axon growth and guidance. We show here that lola mRNAs are generated by alternative trans-splicing of exons sequentially encoded by the same DNA strand. Chromosomal pairing facilitates interallelic trans-splicing,allowing complementation between mutations in the constant and those in the variable exons. We demonstrate that at least one variable exon is transcribed from its own promoter,and trans-spliced to the constant exons transcribed separately.