Artemisinin Biosynthesis Enhancement in TransgenicArtemisia annuaPlants by Downregulation of theβ-Caryophyllene Synthase Gene

作者：Jianlin Chen, Huaming Fang, Yun-Peng Ji, Gaobin Pu, Yanwu Guo, Lili Huang, Zhigao Du, Benye Liu, Hechun Ye, Guofeng Li, Hong Wang · 发表于：Planta Medica · 年份：2011 · DOI：10.1055/s-0030-1271038 · 被引用次数：66 · 研究领域：Plant biochemistry and biosynthesis、Transgenic Plants and Applications、Viral Infectious Diseases and Gene Expression in Insects

Artemisinin is an effective antimalarial drug isolated from the medicinal plant Artemisia annua L. Due to its increasing market demand and the low yield in A. annua, there is a great interest in increasing its production. In this paper, in an attempt to increase artemisinin content of A. ANNUA by suppressing the expression of β-caryophyllene synthase, a sesquiterpene synthase competing as a precursor of artemisinin, the antisense fragment (750 bp) of β-caryophyllene synthase cDNA was inserted into the plant expression vector pBI121 and introduced into A. annua by Agrobacterium-mediated transformation. PCR and Southern hybridization confirmed the stable integration of multiple copies of the transgene in 5 different transgenic lines of A. annua. Reverse transcription PCR showed that the expression of endogenous CPS in the transgenic lines was significantly lower than that in the wild-type control A. annua plants, and β-caryophyllene content decreased sharply in the transgenic lines in comparison to the control. The artemisinin content of one of the transgenic lines showed an increase of 54.9 % compared with the wild-type control. The present study demonstrated that the inhibition pathway in the precursor competition for artemisinin biosynthesis by anti-sense technology is an effective means of increasing the artemisinin content of A. annua plants.