The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome

作者：David A. Sela, Jarrod Chapman, A. Adeuya, Jae‐Han Kim, Fuhong Chen, Terence R. Whitehead, Alla L Lapidus, Daniel S. Rokhsar, Carlito B. Lebrilla, J. Bruce German, Neil P. J. Price, Paul M. Richardson, David A. Mills · 发表于：Proceedings of the National Academy of Sciences · 年份：2008 · DOI：10.1073/pnas.0809584105 · 被引用次数：906 · 研究领域：Probiotics and Fermented Foods、Genomics and Phylogenetic Studies、Gut microbiota and health

Following birth, the breast-fed infant gastrointestinal tract is rapidly colonized by a microbial consortium often dominated by bifidobacteria. Accordingly, the complete genome sequence of Bifidobacterium longum subsp. infantis ATCC15697 reflects a competitive nutrient-utilization strategy targeting milk-borne molecules which lack a nutritive value to the neonate. Several chromosomal loci reflect potential adaptation to the infant host including a 43 kbp cluster encoding catabolic genes, extracellular solute binding proteins and permeases predicted to be active on milk oligosaccharides. An examination of in vivo metabolism has detected the hallmarks of milk oligosaccharide utilization via the central fermentative pathway using metabolomic and proteomic approaches. Finally, conservation of gene clusters in multiple isolates corroborates the genomic mechanism underlying milk utilization for this infant-associated phylotype.