Pre‐mRNA 3′‐end processing complex assembly and function

作者：Serena Leong Chan, Eun‐A Choi, Yongsheng Shi · 发表于：Wiley Interdisciplinary Reviews - RNA · 年份：2010 · DOI：10.1002/wrna.54 · 被引用次数：154 · 研究领域：RNA Research and Splicing、RNA modifications and cancer、RNA and protein synthesis mechanisms

The 3'-ends of almost all eukaryotic mRNAs are formed in a two-step process, an endonucleolytic cleavage followed by polyadenylation (the addition of a poly-adenosine or poly(A) tail). These reactions take place in the pre-mRNA 3' processing complex, a macromolecular machinery that consists of more than 20 proteins. A general framework for how the pre-mRNA 3' processing complex assembles and functions has emerged from extensive studies over the past several decades using biochemical, genetic, computational, and structural approaches. In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges.