Molecular Cloning of the Interleukin-1β Converting Enzyme

作者：Douglas Pat Cerretti, Carl J. Kozlosky, Bruce Mosley, Nicole Nelson, Kirk Van Ness, Teresa A. Greenstreet, Carl J. March, С. Kronheim, Teresa Druck, Linda A. Cannizzaro, Kay Huebner, Roy A. Black · 发表于：Science · 年份：1992 · DOI：10.1126/science.1373520 · 被引用次数：1139 · 研究领域：RNA and protein synthesis mechanisms、Immune Response and Inflammation、Glycosylation and Glycoproteins Research

Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1 beta to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.