Novel Cyanine Dye-Labeled Dideoxynucleoside Triphosphates for DNA Sequencing

作者：R. Scott Duthie, Inta Kalve, Sui‐Bi Samols, Scott C. Hamilton, Inna Livshin, Mahesh Khot, Satyam Nampalli, Shiv Kumar, Carl W. Fuller · 发表于：Bioconjugate Chemistry · 年份：2002 · DOI：10.1021/bc015522y · 被引用次数：24 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、Microfluidic and Capillary Electrophoresis Applications

Single color cyanine dye-labeled (Cy 5.0 and Cy 5.5) dideoxynucleoside-5'-triphosphates, or 'terminators', containing different spacer lengths were synthesized and evaluated for efficacy in DNA sequencing methods using a modified thermally stable DNA polymerase. The single color cyanine dye terminators were formulated into two separate sets of sequencing mixes, one for Cy 5.0 and the other for Cy 5.5, and evaluated on different automated sequencing platforms. Each set of mixes included two pyrimidine terminators with 17-atom linkers and two purine terminators with 10-atom linkers between the dye and the nucleotide. The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods.