A second site specific endonuclease from Thermus thermophilus 111, Tth111II

作者：Takahisa Shinomiya, Mariko Kobayashi, Showbu Sato · 发表于：Nucleic Acids Research · 年份：1980 · DOI：10.1093/nar/8.15.3275 · 被引用次数：36 · 研究领域：Advanced biosensing and bioanalysis techniques、Bacteriophages and microbial interactions、Genomics and Phylogenetic Studies

A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80 degrees C and requires Mg2+ or Mn2+ for endonuclease activity. Tth111II cleaves phi X174RFDNA into 11 fragments and lambda NA into more than 25 fragments. From the 5'-terminal sequences of TthlllII fragments of phi X174RFDNA determined by the two dimensional homochromatography and the survey on nucleotide sequence of phi X174RFDNA, it was concluded that Tth111II recognizes the DNA sequence (see former index) and cleaves the sites as indicated by the arrows.