PHYSICAL AND CHEMICAL STUDIES OF GRAMICIDIN AND SOME IMPLICATIONS FOR THE STUDY OF PROTEINS

作者：R. L. M. Synge · 发表于：Cold Spring Harbor Symposia on Quantitative Biology · 年份：1950 · DOI：10.1101/sqb.1950.014.01.023 · 被引用次数：26 · 研究领域：Genetics, Bioinformatics, and Biomedical Research

In the autumn of 1941, shortly after Dr. A. J. P. Martin and I had obtained our first successful results with partition chromatography at the laboratory of the Wool Industries Research Association in Leeds, we received a visit from Dr. B. C. J. G. Knight. He drew our attention to the then recently published studies of Dubos, Hotchkiss and others on gramicidin, and suggested that this might prove to be an admirable model substance, both for testing our new techniques of amino acid analysis and for assessing more closely the value of the isolation and recognition of products of partial hydrolysis as a means for the study of protein structure (Synge, 1943). Fortunately, we had no idea at the time what complexities lay hidden in gramicidin, and therefore, with Dr. A. H. Gordon, embarked on the study of it. Since that date, the problem of the structure of gramicidin has...