The Neutrophil-Activating Protein (NAP-1) Is Also Chemotactic for T Lymphocytes

作者：Christian Grønhøj, Arthur O. Anderson, Ettore Appella, Joost J. Oppenheim, Kouji Matsushima · 发表于：Science · 年份：1989 · DOI：10.1126/science.2648569 · 被引用次数：1049 · 研究领域：S100 Proteins and Annexins、Cell Adhesion Molecules Research、Immune Response and Inflammation

T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.