Bacterial biogeography of the human digestive tract

作者：Jennifer C. Stearns, Michael D. J. Lynch, Dilani Braziunas Senadheera, Howard Charles Tenenbaum, Michael B. Goldberg, Dennis G. Cvitkovitch, Kenneth Croitoru, Gabriel Moreno‐Hagelsieb, Josh David Neufeld · 发表于：Scientific Reports · 年份：2011 · DOI：10.1038/srep00170 · 被引用次数：400 · 研究领域：Gut microbiota and health、Oral microbiology and periodontitis research、Probiotics and Fermented Foods

We present bacterial biogeography as sampled from the human gastrointestinal tract of four healthy subjects. This study generated >32 million paired-end sequences of bacterial 16S rRNA genes (V3 region) representing >95,000 unique operational taxonomic units (OTUs; 97% similarity clusters), with >99% Good's coverage for all samples. The highest OTU richness and phylogenetic diversity was found in the mouth samples. The microbial communities of multiple biopsy sites within the colon were highly similar within individuals and largely distinct from those in stool. Within an individual, OTU overlap among broad site definitions (mouth, stomach/duodenum, colon and stool) ranged from 32-110 OTUs, 25 of which were common to all individuals and included OTUs affiliated with Faecalibacterium prasnitzii and the TM7 phylum. This first comprehensive characterization of the abundant and rare microflora found along the healthy human digestive tract represents essential groundwork to investigate further how the human microbiome relates to health and disease.