Lymphatic Hyaluronan Flux from Skin Increases during Increased Lymph Flow Induced by Intravenous Saline Loading

作者：Rolf Kåre Reed, Mary I. Townsley, Z. Zhao, Masumi Ishibashi, Torvard C. Laurent, Aubrey E. Taylor · 发表于：International Journal of Microcirculation · 年份：2008 · DOI：10.1159/000178207 · 被引用次数：12 · 研究领域：Lymphatic System and Diseases、Sympathectomy and Hyperhidrosis Treatments、Body Contouring and Surgery

Hyaluronan is a structural component of the interstitial matrix in skin and is catabolized locally in skin as well as by lymphatic removal and subsequent degradation in lymph nodes and liver. The present experiments were designed to evaluate the role of interstitial fluid flux in turnover of hyaluronan in skin by measuring the maximal lymphatic flux of hyaluronan. Lymph flow, total protein concentration, hyaluronan concentration, and flux were measured every 15 min in prenodal lymph from the hind paw in 12 pentobarbital-anesthetized mongrel dogs at normal and increased interstitial fluid flux. An intravenous saline load (15% of body weight during the course of 30 min) was followed by a one-step increase in local venous pressure 30 min later (to a maximum of 50 mm Hg) and maintained at this level for the next 240 min. Lymph flow and hyaluronan concentration during the control period averaged 25.6 +/- (SD) 23.2 (range 4.7-61.9) microliters/min and 8.6 +/- 2.8 (range 2.0-11.6) micrograms/ml, respectively. The hyaluronan concentration fell by 30% during the experimental period, while the lymph flow increased up to ten times above control. Total tissue water increased from 1.73 +/- 0.11 ml/g dry weight during the control period to 1.91 +/- 0.12 ml/g dry weight at the end of the experiment (p < 0.01). The corresponding tissue contents of hyaluronan averaged 3.5 +/- 0.9 and 3.6 +/- 0.6 mg/g dry weight, respectively (p > 0.05). The average lymphatic hyaluronan flux was 8.4 +/- 5.4 mi...