Transcription of the β-like globin genes and pseudogenes of the goat in a cell-free system

作者：Donal S. Luse, Joel R. Haynes, Donald VanLeeuwen, Eric A. Schon, Michael L. Cleary, Steven G. Shapiro, Jerry B. Lingrel, Robert G. Roeder · 发表于：Nucleic Acids Research · 年份：1981 · DOI：10.1093/nar/9.17.4339 · 被引用次数：65 · 研究领域：Vector-Borne Animal Diseases、Heat shock proteins research、Wheat and Barley Genetics and Pathology

We have examined the transcription in a cell-free system of all the members of a highly regulated gene family, the beta-like hemoglobin genes of the goat. These five genes, which code for embryonic, fetal, juvenile, and adult beta-globin proteins, are all transcribed to roughly the same extent in the in vitro system. In all cases initiation of transcription is accurate. However, two goat beta-globin pseudogenes, as well as several artificially constructed deletion mutants, are not transcribed in vitro. A common feature of the transcriptionally inactive genes is the lack of an AT-rich consensus sequence just upstream of the presumptive initiation site.