Isolation of Microtubule Protein from Cultured Mouse Neuroblastoma Cells

作者：Joanna B. Olmsted, Kathryn E. Carlson, Robert J. Klebe, F.H. Ruddle, Joel L. Rosenbaum · 发表于：Proceedings of the National Academy of Sciences · 年份：1970 · DOI：10.1073/pnas.65.1.129 · 被引用次数：318 · 研究领域：Glycosylation and Glycoproteins Research、14-3-3 protein interactions、Carbohydrate Chemistry and Synthesis

The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.