Cloning of sucrase genes fromStreptococcus mutansin bacteriophage lambda

作者：Roy R. B. Russell, P Morrissey, Gordon Dougan · 发表于：FEMS Microbiology Letters · 年份：1985 · DOI：10.1111/j.1574-6968.1985.tb00981.x · 被引用次数：20 · 研究领域：Glycosylation and Glycoproteins Research、Bacteriophages and microbial interactions、Enzyme Production and Characterization

Chromosomal DNA from Streptococcus mutans strain Ingbritt (serotype c) was cloned into the bacteriophage λ replacement vector L47.1. The bank of recombinant phage was screened for the presence of plaques in which sucrose-hydrolysing (sucrase) activity was expressed. Two distinct sucrase-expressing recombinants were identified. In one type, designated scr, the product is an invertase-like enzyme. This enzyme was purified from lysates of recombinants and shown to have an apparent Mr of 59000. The second class of recombinants was found to express a glucosyltransferase, identical in size, as well as in enzymatic and antigenic properties, to the previously described product of gtfA.