Efficient methods for attaching non-radioactive labels to the 5′ ends of synthetic oligodeoxyribonucleotides

作者：Sudhir Agrawal, Chris C. Christodoulou, Michael John Gait · 发表于：Nucleic Acids Research · 年份：1986 · DOI：10.1093/nar/14.15.6227 · 被引用次数：222 · 研究领域：Biotin and Related Studies、Click Chemistry and Applications、Advanced biosensing and bioanalysis techniques

The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.