Effect of Nano red elemental selenium on GPx activity of broiler chick kidney cellsin vitro

作者：Baohua Xu, XU Zi-rong, Xia Mei-sheng, HU Cai-hong, Deng Yuesong, Xiong Li · 发表于：Wuhan University Journal of Natural Sciences · 年份：2003 · DOI：10.1007/bf02903692 · 被引用次数：10 · 研究领域：Selenium in Biological Systems、Rabbits: Nutrition, Reproduction, Health、Animal Nutrition and Physiology

A new selenium source, Nano red elemental selenium (Nano-Se) was used to study the effect on the GPx activity of broiler chick kidney cells (BCKC) in vitro , Sodium selenite (Na 2 SeO 3 ) and seleno-1-methionine (Se-Met) were used as the controls. The results showed that the effects of three kinds of Se forms on the GPx activity of BCKC were accordant ( p >0.05) compared with each other at 0.01. 0.05 and 0.10 μmol/L Se concentrations treatments. In the range of 0.00–0.10 μmol/L Se concentrations, the GPx activity increased with elevation of Se concentrations in medium. For the three kinds of Se forms, the GPx activity reached the climax at 0.10 μmol/L Se concentration. At 0.20 and 0.30 μmol/L Se concentrations, the influnces of three kinds of Se forms were not accordant with one another. For Nano-Se, the GPx activity at 0.20 and 0.30 μmoi/L Se concentrations remained the same as that at 0.10 μmol/L Se concentration treatment. For Se-Met, the GPx activity at 0.20 μmol/L Se concentration treatment remained the same with 0.10 μmol/L treatment; the GPx activity at 0.30 μmol/L Se concentration treatment was declined significantly ( p <0.05) compared with 0.10 or 0.20 μmol/L treatment. For Na 2 SeO 3 , the GPx activity falled gradually with Se concentration increasing from 0.10 μmol/L to 0.30 μmol/L, and at 0.30 μmol/L Se concentration treatment, the GPx activity was less than the original of BCKC. The results implicated, on the GPx activity of BCKC in vitro , the ranking of width ...