A Gaijin-like miniature inverted repeat transposable element is mobilized in rice during cell differentiation

作者：Haitao Dong, Lu Zhang, Kang-Le Zheng, Haigen Yao, Jack L.‐Y. Chen, Feng-Chi Yu, Xiaoxing Yu, Bizeng Mao, Dong Zhao, Jian Yao, Debao Li · 发表于：BMC Genomics · 年份：2012 · DOI：10.1186/1471-2164-13-135 · 被引用次数：23 · 研究领域：Chromosomal and Genetic Variations、Plant Molecular Biology Research、Plant Reproductive Biology

BACKGROUND: Miniature inverted repeat transposable element (MITE) is one type of transposable element (TE), which is largely found in eukaryotic genomes and involved in a wide variety of biological events. However, only few MITEs were proved to be currently active and their physiological function remains largely unknown. RESULTS: We found that the amplicon discrepancy of a gene locus LOC_Os01g0420 in different rice cultivar genomes was resulted from the existence of a member of Gaijin-like MITEs (mGing). This result indicated that mGing transposition was occurred at this gene locus. By using a modified transposon display (TD) analysis, the active transpositions of mGing were detected in rice Jiahua No. 1 genome under three conditions: in seedlings germinated from the seeds received a high dose γ-ray irradiation, in plantlets regenerated from anther-derived calli and from scutellum-derived calli, and were confirmed by PCR validation and sequencing. Sequence analysis revealed that single nucleotide polymorphisms (SNPs) or short additional DNA sequences at transposition sites post mGing transposition. It suggested that sequence modification was possibly taken place during mGing transposition. Furthermore, cell re-differentiation experiment showed that active transpositions of both mGing and mPing (another well studied MITE) were identified only in regenerated plantlets. CONCLUSIONS: It is for the first time that mGing active transposition was demonstrated under γ-ray irradiation...