Isolation and analysis of ketoconazole resistant mutants ofSaccharomyces cerevisiae

作者：P. F. Watson, M. E. Rose, Steven L. Kelly · 发表于：Medical Mycology · 年份：1988 · DOI：10.1080/02681218880000231 · 被引用次数：45 · 研究领域：Fungal Biology and Applications、Microbial Natural Products and Biosynthesis、Steroid Chemistry and Biochemistry

Nine mutants of Saccharomyces cerevisiae which are resistant to ketoconazole, have been isolated and characterized. In each case the mutation is nuclear in origin and allelic to a previously described mutation, erg3, which gives rise to a block in the delta 5-6 desaturation step of ergosterol biosynthesis. The significance of this second site mutation to the point of inhibitory action of ketoconazole, that is the P-450-mediated C-14 demethylation of lanosterol, is discussed.