The Use of pHluorins for Optical Measurements of Presynaptic Activity

作者：Sethuraman Sankaranarayanan, Dino A. De Angelis, James E. Rothman, Timothy A. Ryan · 发表于：Biophysical Journal · 年份：2000 · DOI：10.1016/s0006-3495(00)76468-x · 被引用次数：634 · 研究领域：Advanced Fluorescence Microscopy Techniques、Photoreceptor and optogenetics research、Cellular transport and secretion

Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.