Expression and regulation of osteopontin in chronic rhinosinusitis with nasal polyps

作者：W.‐L. Liu, Hong Zhang, Y Zheng, Huijuan Wang, F.‐H. Chen, Ling Xu, Yi Wei, Yueqi Sun, Jianbo Shi, H.‐B. Li · 发表于：Clinical & Experimental Allergy · 年份：2014 · DOI：10.1111/cea.12320 · 被引用次数：27 · 研究领域：Sinusitis and nasal conditions、Bone and Dental Protein Studies、Asthma and respiratory diseases

BACKGROUND: Osteopontin (OPN) has been proposed to be associated with airway inflammation including asthma and chronic rhinosinusitis with nasal polyps (CRSwNP). This study sought to evaluate the expression and regulation of the OPN in CRSwNP patients. METHODS: Nasal polyp (NP) tissues and normal tissues were collected from 30 CRSwNP patients and 16 control subjects. The expression and regulation of OPN, as well eosinophil (EOS) accumulation and activation, were examined in nasal tissues using DNA microarray, immunohistochemical (IHC), immunofluorescent (IF) staining and qPCR analysis. Moreover, the regulation of OPN in nasal epithelial cells and its effects on migration and activation of EOS were evaluated in vitro using flow cytometry, ELISA, qPCR and Western blot, etc. RESULTS: DNA microarray analysis identified OPN as one of the 19 upregulated genes in polyp tissues. The mean number of OPN(+) cells in polyp tissues was found to be significantly increased compared with the normal controls (P < 0.01), and OPN(+) cells in polyp tissues significantly correlated with tissue eosinophilia [major basic protein (MBP)(+) cells; r = 0.51, P < 0.01]. Accordingly, the mRNA and protein levels of OPN in NP tissues were significantly higher than those in normal controls (P < 0.01). Poly I:C, flagellin and TLR-9 agonist CpG ODN, as well as TNF-α, IFN-γ, IL-6, IL-17A and TGF-β, significantly increased OPN mRNA expression in cultured PECs and NECs (P < 0.05). Recombinant human OPN significa...